Cdk2 Antibody Cell Signaling

Cdk2 cell division protein kinase 2 cyclin dependent kinase 2 cdc2 related protein kinase cell devision kinase 2 p33 protein kinase p33 cdk2 immunogen.

Cdk2 antibody cell signaling.

Green signal from target ab32147 observed at 34 kda lanes 3 and 4. Inhibitory phosphorylation occurs on thr14 and tyr15 1. Inhibitory phosphorylation occurs on thr14 and tyr15 1. Lane 1 is 10 input.

Antibodies are purified by protein a and peptide affinity chromatography. Wild type hap1 cell lysate 20 µg lanes 2 4 and 6. Merged red and green signal. To prepare 10 ml add 30 µl triton x 100 to 10 ml 1x pbs.

Lanes 1 3 and 5. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding thr160 of human cdk2. Ab32147 was shown to specifically react with cdk2 when cdk2 knockout samples were used. Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.

Phospho cdk2 thr160 antibody detects endogenous levels of cdk2 only when phosphorylated at threonine 160. Mix well then add 0 1 g bsa mix. Immunoprecipitation of cdk12 from pc 3 cell extracts using normal rabbit igg 2729 lane 2 or cdk12 antibody lane 3. The antibody weakly cross reacts with cdc2 phosphorylated at thr161.

An antibody shouldn t be one of the variables in your experiment. It does not cross react with other phosphorylated cyclin dependent kinases. Antibody dilution buffer 1x pbs 1 bsa 0 3 triton x 100. Cdk2 knockout hap1 cell lysate 20 µg lanes 1 and 2.

Recommended fluorochrome conjugated anti rabbit secondary antibodies. Find out why customers rank cst highest for antibody specificity and sensitivity. Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery. Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.

Anti rabbit igg h l f ab 2 fragment alexa fluor 488 conjugate 4412. Western blot analysis was performed using cdk12 antibody. Kinase activity associated with cyclin a cdc2 is found only in g2 phase. Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery.

Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery. Red signal from loading control ab8226 observed at 42 kda lanes 5 and 6.