For critical work we suggest that you fix and stain cultures and then carefully calculate the actual growth surface area.
Cell culture plate surface area.
40 stack with 25440 cm 2 cell growth area.
Cell culture plate multi well plates multiple well plates multiwell plates tissue culture plates.
The available surface area.
Corning costar tc treated multiple well plates size 6 wells clear polystyrene plate flat bottom case of 50 individually wrapped sterile lid.
Cell culture plate multi well plates multiple well plates multiwell plates tissue culture plates.
Available with patented cellbind surface for optimal cell attachment costar tc treated surface or non treated.
1 stack with 636 cm 2 cell growth area.
Actual cell yields can.
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5 stack with 3180 cm 2 cell growth area.
Growth area of tissue culture plates and dishes growth area cm 2.
Chart showing surface area seeding density cells at confluency and volumes of versene trypsin and medium for various culture dishes plates and flasks.
Plates have flat bottoms with round wells unless otherwise indicated.
Developed by corning scientists this patented technology u s.
In general at least 1 x 105 cells cm2 can be produced when growing cells as attached monolayers in culture.
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Surface areas of cell culture vessels the following are the surface areas of various tissue culture dishes or plates and their relative size to a 35 mm dish.
The corning cellstack cell culture chambers are designed for scale up cell culture applications regardless of whether your cells grow attached or in suspension.
The thermo scientific nunclon delta cell culture surface treatment promotes maximum adhesion for a broad range of cell types.
The nunclon delta surface is a fully synthetic energy treated surface that makes the otherwise very hydrophobic polystyrene surface more hydrophilic thus facilitating cell attachment and growth.
Media volume ml maximum volume ml plates 35 mm.
6 617 152 uses a microwave plasma process for treating the culture surface.
Available in five sizes.
Dish diameter actual area calculated area relative to 35 mm dish 35 mm 10 cm2 9 6 cm2 1 60 mm 220 cm 28 cm2 2 100 mm 60 cm 2 78 5 cm 6.
2 stack with 1272 cm 2 cell growth area.
10 stack with 6360 cm 2 cell growth area.
This process improves cell attachment by incorporating significantly more oxygen into the cell culture surface rendering it more hydrophilic.
The average cell yields used here are based on this number.